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Western blot analysis of extracts of various cell lines, using CDC42 antibody (A1188) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
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Immunohistochemistry of paraffin-embedded rat testis using CDC42 antibody (A1188) at dilution of 1:100 (40x lens).
CDC42 Rabbit pAb
Catalog No：AB-A1188
Manufacturer：ABclonal
Synonyms：CDC42;CDC42Hs;G25K;TKS
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | CDC42 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 25-191 of human CDC42 (NP_001782.1). |
| Gene ID: | 998 |
| Swiss prot: | P60953 |
| Molecular weight: | 21kDa |
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