Western blot analysis of extracts of various cells, using Alpha-Fetoprotein (Alpha-Fetoprotein (AFP)) antibody (A11865) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Alpha-Fetoprotein (AFP) Mouse mAb
Catalog No:AB-A11865
Manufacturer:ABclonal
Synonyms:AFPD;FETA;HPAFP;AFP
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Mouse
Conjugate:No
€65,00 – €245,00
Product Name: | Alpha-Fetoprotein (AFP) Mouse mAb |
Category: | Monoclonal Antibodies |
Description: | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. |
Immunogen: | Recombinant protein of human Alpha-Fetoprotein (Alpha-Fetoprotein (AFP)) |
Gene ID: | 174 |
Swiss prot: | P02771 |
Molecular weight: | 68kDa |
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