Western blot analysis of extracts of various cell lines, using HLA-DRA antibody (A11787) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
HLA-DRA Rabbit pAb
Catalog No:AB-A11787
Manufacturer:ABclonal
Synonyms:HLA-DRA;HLA-DRA1;major histocompatibility complex;class II;DR alpha
Applications:WB,IHC
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | HLA-DRA Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-216 of human HLA-DRA (NP_061984.2). |
Gene ID: | 3122 |
Swiss prot: | P01903 |
Molecular weight: | 28kDa |
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