Western blot analysis of extracts of various cell lines, using STAT5A antibody (A11779) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
STAT5A Rabbit pAb
Catalog No:AB-A11779
Manufacturer:ABclonal
Synonyms:MGF;STAT5;STAT5A
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€100,00 – €370,00
Product Name: | STAT5A Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human STAT5A (NP_003143.2). |
Gene ID: | 6776 |
Swiss prot: | P42229 |
Molecular weight: | 86kDa/90kDa |
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