Western blot analysis of extracts of various cell lines, using EpCAM antibody (A1177) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
EpCAM Rabbit pAb
Catalog No:AB-A1177
Manufacturer:ABclonal
Synonyms:EPCAM;DIAR5;EGP-2;EGP314;EGP40;ESA;HNPCC8;KS1/4;KSA;M4S1;MIC18;MK-1;TACSTD1;TROP1
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | EpCAM Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human EpCAM (NP_002345.2). |
Gene ID: | 4072 |
Swiss prot: | P16422 |
Molecular weight: | 34kDa |
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