Western blot analysis of extracts of various cell lines, using SEMA3F antibody (A11713) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
SEMA3F Rabbit pAb
Catalog No:AB-A11713
Manufacturer:ABclonal
Synonyms:SEMA3F;SEMA-IV;SEMA4;SEMAK
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | SEMA3F Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the semaphorin III family of secreted signaling proteins that are involved in axon guidance during neuronal development. The encoded protein contains an N-terminal Sema domain, an immunoglobulin loop and a C-terminal basic domain. This gene is expressed by the endothelial cells where it was found to act in an autocrine fashion to induce apoptosis, inhibit cell proliferation and survival, and function as an anti-tumorigenic agent. Alternative splicing results in multiple transcript variants encoding different isoforms. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 686-785 of human SEMA3F (NP_004177.3). |
Gene ID: | 6405 |
Swiss prot: | Q13275 |
Molecular weight: | 85kDa/88kDa |
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