Western blot analysis of extracts of HeLa cells, using PRIM2 antibody (A11673) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
PRIM2 Rabbit pAb
Catalog No:AB-A11673
Manufacturer:ABclonal
Synonyms:PRIM2;PRIM2A;p58
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PRIM2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. Alternative splicing of this gene results in multiple transcript variants. This gene has a related pseudogene, which is also present on chromosome 6. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human PRIM2 (NP_000938.2). |
Gene ID: | 5558 |
Swiss prot: | P49643 |
Molecular weight: | 19kDa/58kDa |
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